Meta-Analysis-Assisted Detection of Gravity-Sensitive Genes in Human Vascular Endothelial Cells

Gravity affects the function and maintenance of organs, such as bones, muscles, heart. Several studies have used DNA microarrays to identify genes with altered expressions in response gravity. However, it is technically challenging combine results from various microarray datasets because their different data structures. We hypothesized that possible common changes gene expression obtained under conditions methods. In this study, we grouped homologous perform a meta-analysis multiple vascular endothelial cell skeletal muscle datasets. According t-distributed stochastic neighbor embedding (t-SNE) analysis, pattern cells formed specific clusters. also identified candidate responded Further, exposed human umbilical vein (HUVEC) simulated microgravity (SMG) using clinostat measured levels genes. Gene analysis qRT-PCR revealed level prostaglandin (PG) transporter SLCO2A1 decreased microgravity, consistent Furthermore, direction gravity affected , buttressing finding its was by These suggest may help new target previously overlooked individual analyses.